Thin-layer chromatography (TLC) involves coating a suitable stationary phase onto a glass plate, plastic, or aluminum substrate to form a uniform thin layer. After spotting and development, the ratio shift (Rf) of the chromatogram is compared with the ratio shift (Rf) of the chromatogram obtained by the same method for a suitable reference substance. This method is used for drug identification, impurity detection, or content determination. TLC is an important experimental technique for the rapid separation and qualitative analysis of small amounts of substances and is also used to track reaction progress.